England’s talisman was brought down early in the second half and though he picked himself off the turf, his ankle will now be assessed.
England captain Harry Kane will go for a scan on his ankle ahead of the Three Lions’ next World Cup encounter against the United States.
Kane was brought down in a heavy challenge from Mortez Pouraliganji three minutes into the second half of England’s 6-2 thrashing of Iran on Monday.
After some treatment, the forward recovered and continued until the 75th minute when he was replaced by Newcastle forward Callum Wilson.
Kane was seen limping off after the game with his right ankle in a sling, although manager Gareth Southgate appeared unconcerned about the striker’s fitness, saying he had been substituted because the game had already been won.
But the Tottenham star will now have his injury assessed, raising concerns over the talisman’s availability for England’s Group B second leg against the USA on Friday.
The 29-year-old registered two assists before making way for Wilson as England ran riot against Iran and would be a big miss for Southgate if his ankle injury proves serious enough to rule him out.
Kane is just two goals away from equaling Wayne Rooney’s 53 goals as the Three Lions’ all-time top scorer.